Access to Innovative Technologies --Advanced Light Microscopy (WP7)

 

CORRELATIVE LIGHT-ELECTRON MICROSCOPY

 

Facility Nr. 24
Cell Microscopy Centre, University Medical Centre Utrecht
Heidelberglaan 100, 3584 CX Utrecht, The Netherlands
http://www.cmc-utrecht.nl/

Contact: j.klumperman@umcutrecht.nl

Offered Technology (ies): Correlative Light-Electron Microscopy; live cell imaging – immunoEM; thin section LM – immunoEM; membrane trafficking

Together with

Department of Molecular Cell Biology, Section Electron Microscopy, LeidenUniversity Medical Center, Leiden, The Netherlands
http://electronmicroscopy.lumc.nl/

Contact: A.J.Koster@lumc.nl

Offered Technology (ies): Correlative Light-Electron Microscopy; Fluorescence – cryoET; iLEM (integrated LM-SEM); technique development

 

Facility Nr. 25
Wolfson Bioimaging Facility, Universityof Bristol, School of Biochemistry, Medical Sciences, University Walk, Bristol BS8 1TDUnited Kingdom
www.bristol.ac.uk/biochemistry/research/pv.html

Contact: p.verkade@bristol.ac.uk

Offered Technology (ies): Correlative Light-Electron Microscopy; Live cells –HPF-FS-electron tomography; Cell Biology

 

Facility Nr. 26
Telethon Electron Microscopy Core Facility, Telethon Institute of Genetics and Medicine Napoli, Via Pietro Castellino, 111, 80131 - Napoli, Italy
www.tigem.it

Contact: luini@tigem.it; polish@tigem.it;

Offered Technology (ies): Correlative Light-Electron Microsocpy; Live cell imaging – pre-embedding – TEM; membrane trafficking; 

 

Facility Nr. 27
Imaging Center - IGBMC; IGBMC-CERBM Strasbourg-Illkirch France
http://www.ici.u-strasbg.fr

Contact: Yannick Schwab (member INSTRUCT); yschwab@igbmc.fr

Offered Technology (ies): Live imaging, F-techniques; HPF- FS- Lowicryl/EPON; Immuno EM, 3D EM; Developmental and Cell Biology.

HIGH-THROUGHPUT - HIGH-DEFINITION MICROSCOPY

 

Facility Nr. 28
Advanced Light Microscopy Facility (ALMF); EMBL Heidelberg Meyerhofstr. 1 D – 69117 Heidelberg, Germany
http://www.embl.de/almf/almf_services/

Contact: almf@embl.de

Offered Technology(ies)
High throughput/content light microscopy
Technical details:see http://www.embl.de/almf/almf_services/

Available Resources
Instruments
Technical assistance to assay development
Sample preparation
Run instrument
Data management processing and analysis
siRNA arrays

 

Facility Nr. 29
Multimodal Imaging Core, Kuopio, University of Eastern Finland; A. I. Virtanen Institute, Biocenter Kuopio, University of Eastern Finland, Kuopio, Finland
www.uef.fi/aivi/muic

Contact: aila.seppanen@uef.fi 
mjczmjc@gmail.com

Offered Technology(ies)
High throughput microscopy
Technical details:see www.uef.fi/aivi/muic

Recommendations for compatible sample preparation and assay suitability; some automated sample preparation automated on site, image acquisition, image segmentation and analysis, some data mining.

Available Resources
Instruments
Technical assistance to run instrument
Methodological setup (e.g. design of study protocol)
Training in infrastructure use
Data processing and analysis

 

Facility Nr. 30

WIS-AUTOSCOPE, Weizmann Institute of Science, Rehovot, 76100, Israel.
http://www.weizmann.ac.il/mcb/Geiger/

Contact:Benny.geiger@weizmann.ac.il; b.morgenstern@weizmann.ac.il

Offered Technology(ies)
High-throughput Microscopy:
HTS screening
HCS screening
Image segmentatopn
Image quantification

Technical details: Instrumentation: WiScan prototype, WiScan Argus, WiScan Hermes (Idea BioMedical), Liquid handling robotics

Available resources
Instruments
Tech Assistance
Computation infrastructure
Wet Lab
Seminar rooms
Housing facilities

 

SUPER-RESOLUTION MICROSCOPY

Facility Nr. 31
Department of Nano-Biophotonics MPI Göttingen, Facility for Innovative Light Microscopy, Göttingen, Germany.
http://www.4pi.de/

Contact: shell@gwdg.de

Offered Technology(ies)
STED (multicolor, also in combination with 4PI)
RESOLFT
GSDIM (multicolor, also in combination with 4PI)
PALM, STORM
STED FCS (multicolor)
FLIM
FRAP, FRET
Single molecule imaging

Technical details: Home-build STED, RESOLFT, GSDIM and other SMS microscope setups for superresolution imaging. Multiple setups life-cell compatible

Available Resources
Instruments
Technical assistance to run instruments
Methodological setup
Probe preparation
Wet lab space
Data processing and analysis

    Facility Nr. 32
Science for life laboratory Stockholm, Sweden
http://www.scilifelab.se/index.php?content=alm

TOGETHER WITH

Turku BioImaging, Turku, Finland
http://www.btk.fi/cell-imaging/instrumentation/confocal-microscopes/leica-tcs-sp5-sted/

Contact: pekka.hanninen@utu.fi  ; brismar@kth.se;

Offered Technology(ies)
STED (dual color)
CW-STED
Multiphoton
FRAP, FRET
FCS
SPT in TIRF-Epi mode (QDots, dyes, XFP)

Technical details: Leica SP5 commercial 2-channel instrument with pulsed illumination/STED. Home build STED system expertise. Set-up of instrumentation.

Available Resources
Instruments
Technical assistance to run instruments
Methodological setup
Probe preparation
Wet lab space
Data processing and analysis

Facility Nr. 33
BordeauxImaging Center, Center for Functional Genomics of Bordeaux; France
http://www.bic.u-bordeaux2.fr/

Contact: bic@u-bordeaux2.fr

Offered Technology(ies)
STED (dual color)
PALM, STORM
FLIM-FRET
Single molecule imaging techniques
Photothermal Heterodyne Imaging

Technical details: Set-up of instrumentation. Association with electro-physiologyNanoparticle coupling.

Available Resources
Instruments
Technical assistance to run instruments
Probe preparation
Wet lab space
Data processing and analysis
Animal facilities (rodents)
Bio-safety L2
 

Facility Nr. 34

SLN@BCN: BarcelonaSuper-Resolution Light Microscopy Alliance, ICFO-The Institute of Photonic Sciences. MediterraneanTechnology Park, 08860 Castelldefels (Barcelona), Spain.
http://www.icfo.es/

Contact: maria.garcia-parajo@icfo.es

Offered Technology(ies)
TIRF
STED (dual color)
PALM, STORM
Non-linear (SHG,CARS)
Single molecule imaging

Technical details: Set-up of instrumentation. Dual color SPT in TIRF-Epi mode (QDots, dyes, XFP). SM set-up adapted for FLIM, FRET, FCS on living cells.

Available Resources
Instruments
Technical assistance to run instruments
Methodological setup
Probe preparation
Wet lab space
Data processing and analysis

 

FUNCTIONAL IMAGING

Facility Nr. 35
European Laboratory for non linear spectroscopy (LENS)
www.lens.unifi.it/bio

Contact:francesco.pavone@unifi.it or pavone@lens.unifi.it

Offered Technology(ies)
Single Plane Illumination Microscopy (SPIM)
Multiphoton Systems
Fluorescence Lifetime Imaging Microscopy (FLIM)
Non-linear Microscopy Techniques (e. g. CARS, SHG, THG and SRS)
Single Molecule Imaging Techniques

Technical details: see http://www.biophotonicslab.eu; Consultation on sample prep; Setting up instrumentation;data acquisition, data processing and analysis

Available resources
Instruments
Technical assistance to run instrument
Methodological setup
Data analysis and setup
Training seminar room

 

Facility Nr. 36
Photonics Group, 606 Blackett Building; Imperial College London, South Kensington Campus, London, SW7 2AZ, UK
http://www3.imperial.ac.uk/photonics

Contact:photonics@imperial.ac.uk

Offered Technology(ies)

Fluorescence Lifetime Imaging Microscopy (FLIM) implemented in microscopes, endoscopes, tomography and multiwell plate readers
High-throughput FLIM Microscopy
FLIM Optical Projection Tomography (OPT)

Technical details: Automated wide-field and optically sectioned(Nipkow disc) FLIM microscopes and plate readers; Wide-field time-gated detection for rapid FLIM; Tunable ultrafast supercontinuum excitation from u.v. to NIR; FLIM OPT of mm-cm samples, Confocal FLIM endomicroscopy

Available Resources
Instruments
Technical assistance to run instrument
Methodological setup (e.g. design of study protocol)
Training in infrastructure use
Wet lab space
Server Space
Data processing and analysis
Training seminar room
Bio-safety L2

 

Facility Nr. 37
University of Konstanz Bioimaging Center; University of Konstanz, Dept. of Biology, D-78464 Konstanz
www.bioimaging-center.uni-konstanz.de/

Contact:bioimaging@uni-konstanz.de

Offered Technology(ies)
Functional Imaging of Living Cells (e.g. FRAP, FRET)
Multiphoton Systems

Technical details: Two-Color Nonlinear Photoperturbation with Tunable Femtosecond Fiber Lasers; Applications: DNA repair and DNA-damage induced nuclear protein dynamics; Expertise in novel femtosecond light sources for biophotonics (see http://www.uni-konstanz.de/quantum-electronics)
Custom image processing and analysis with KNIME (see www.incide.uni-konstanz.de)

Available Resources
Instruments
Technical assistance to run instrument
Probe preparation
Wet lab space
Data processing and analysis

 

Facility Nr. 38
MARS, Centre de Biochimie Structurale
29 rue de Navacelles. 34090 Montpellier, France
And Montpellier RioImaging
www.cbs.cnrs.fr/mars

Contact: Catherine Royer (royer@cbs.cnrs.fr) Edouard.bertrand@igmm.cnrs.fr

 

Offered Technology(ies)
Fluorescence Fluctuation Spectroscopy (FFS),
Single Molecule Imaging Techniques,
Multiphoton Systems,
Photoactivated Localization Microscopy (PALM),
Total Internal Reflection Fluorescence Microscopy (TIRF)
Single particle tracking (SPT)
High throughput colocalization imaging

Technical details: Consultation on sample prep; data acquisition, data processing and analysis, see www.cbs.cnrs.fr/mars

Available resources
Instruments
Technical assistance to run instrument
Methodological setup (e.g. design of study protocol)
Wet Lab Space
Data processing and analysis