Home > Access to Molecular Imaging Technologies (WP8)

Access to Molecular Imaging Technologies (WP8)

 

MESOSCOPIC IMAGING: optical methods for model organisms and embryos

 

Facility Nr. 39
Laboratory James Sharpe (not a facility), EMBL-CRG Systems Biology Unit, Centre for Genomic Regulation, Barcelona, Spain.
www.crg.es/jamessharpe

Contact: james.sharpe@crg.eu

Offered Technology (ies):Optical Projection Tomography (OPT)

Available resources
Instruments
Technical assistance to run instrument
Training in infrastructure use
Wet lab space
Data processing and analysis

 

Facility Nr. 40
Laboratory of Jan Huisken (not a facility), Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG), Dresden, Germany.
http://www.mpi-cbg.de/huisken

Contact: huisken@mpi-cbg.de

Offered Technology (ies) : Selective Plane Illumination Microscopy (Two-color, high-speed SPIM)

Available resources:
Instruments
Animal facilities (zebrafish)
Technical assistance to run instrument
Training in infrastructure use
Wet lab space
Data processing and analysis

INTEGRATED MOLECULAR IMAGING for small animal models

 

Facility Nr. 41
European Institute for Molecular Imaging (EIMI), Westfälische Wilhelms-Universität
Mendelstr. 11, Münster, GERMANY
www.uni-muenster.de/EIMI/

Contact: ahjacobs@uni-muenster.de

Offered Technology (ies)
Expertise on multimodality imaging: µCT / µPET / µSPECT / µMRI / µUS / BLI / FRI/FMT

Available Resources
Instruments and Animal facilities
Data processing and analysis
Methodological setup (e.g. design of study protocol and standard operator procedures) Technical assistance and training in infrastructure use

 

Facility Nr. 42
Radboud preclinical imaging Centre (PRIME), Radboud University Nijmegen Medical Centre, PO Box 9101, 6500 HB Nijmegen, The Netherlands
www.umcn.nl/PRIME

Contact: o.boerman@nucmed.umcn.nl

Offered Technology (ies)
µPET/CT
µSPECT
µMRI
Multiphoton
(MPM) Bioluminescence (BLI)

Technical Details
MRI (11.7 T and 7 Tesla), µPET/CT, µSPECT/CT, NIR/IR; MPM/FLIM, BLI, (all installed next to each other in one facility)

Available Resources
Animal facilities
Animal preparation
Data processing and analysis
Housing
Probe preparation
Technical assistance to run instrument
Wet lab space
Methodological setup (study design, animal model, etc.)

A

Facility Nr. 43
Biocenter Finland – In vivo imaging infrastructure:

A) TurkuPET Centre, University of Turku and Turku University Hospital, Finland
www.pet.fi
Contact: juhani.knuuti@utu.fi
Offered Technology (ies):
MR-PET
PET-CT
SPECT-CT
µCT
µPET
µUS

B) Biomedical Imaging Unit, A.I Virtanen Institute, University of Kuopio, POBox1627, 70211 Kuopio, Finland
http://www.uef.fi/aivi/biu
Contact: olli.grohn@uef.fi
Offered Technology (ies):
µMRI 
µSPECT/CT
µUS

Technical Details for A) and B): 
Expertise on multimodality molecular imaging in small and large animals and humans; Expertise especially in MRI and MRS as well as in multimodality imaging.

Available resources for A) and B):
Animal facilities
Animal preparation
Data processing and analysis
Housing facilities
Instruments
Methodological setup (e.g. design of)
Technical assistance to run instrument
Training in infrastructure use
Training seminar room
Training workstations
Wet lab space
Probe preparation

 

Facility Nr. 44
Lokey Pre-clinical Research Facility, Weizmann Institute of Science, Rehovot Israel, http://www.weizmann.ac.il/vet/IC/

Contact: michal.neeman@weizmann.ac.il

Offered Technology (ies)
Expertise on multimodality imaging:
µCT
µMRI
µUS
BLI
FRI/FMT
Multiphoton
Intravital endoscopy

Available resources
Animal facilities
Animal preparation
Data processing and analysis
Housing facilities
Instruments
Methodological setup (e.g. design of study protocol and standard operator procedures) Methodological setup (e.g. design of study protocol)
Technical assistance to run instrument

PROBES FOR PET, SPECT, and MRI

 

Facility Nr. 45
Lab. of Tracer Development and Radiopharmacology of the IBFM-CNR,
IBFM-CNR, Milan, Italy.
http://www.ibfm.cnr.it

Contact: moresco.rosamaria@hsr.it

Offered Technology (ies): Positron Emission Tomography (µPET) combined with Single Photon Emission Computed Tomography (µSPECT); Computed Tomography (µCT);

Technical Details:
3 Cyclotrons and 2 group of Radiochemistry Laboratories for preclinical studies and for clinical studies (according to regulatory requirements) located one at San Raffaele Hospital and the other at Fondazione Technomed, Nuclear Medicine Department  of the University of Milan Bicocca. The IBFM of CNR operates and has access, through a formal collaboration with preclinical and clinical facilities and personnel belonging to San Raffaele Hospital (Nuclear Medicine Department)and the Nuclear Medicine Department of the Fondazione Technomed of the University of Milan Bicocca.

Main resources: isotopes production including radio-metals; development and validation of radiopharmaceuticals in selected animal models of diseases; translation to clinical phase I and advanced studies; images acquisition,  processing and analysis; protocols for characterization of preclinical model of disease and drug-development.

Available resources:
Probe preparation (PET and SPECT)
Animal models preparation and animal facilities (small rodents) for probe validation
Methodological setup (e.g. design of study protocol)
Data processing and analysis
Translation of probes to clinical validations

TOGETHER WITH

Experimental imaging center (EIC), Imaging Research Center, San Raffaele Scientific Institute, DIBIT-1, Room 19, 1st Floor, Via Olgettina 58, 20132 Milano, Italy.
www.sanraffaele.org/home/ricerca/divisioni_centri_istituti_programmi/Imaging/index.html

 

Contact: carlo.tacchetti@hsr.it

Offered Technology (ies):
Magnetic Resonance Imaging (MRI)
Color
Doppler
Ultrasound
(CDUS)

Technical Details:
MRI: 7T 30 cm bore (Bruker Biospec) preclinical scanner equipped with both body and neuro coils
CDUS: Vevo 2100 Visualsonics equipped with high-frequency linear probe for general, cardiac, abdominal CDUS without and with contrast agents.
Experience in tracing Gd labeled cells in vivo 

Available resources:
Animal facilities
Biobanking, biological material storage and processing
Data processing and analysis
Housing facilities
Instruments
Methodological setup (e.g. design of study protocol and standard operator procedures) Pharmacovigilance

 

Facility Nr. 46
Molecular Imaging Center, University of Torino
Molecular Biotechnology Center (MBC), Via Nizza 52 - 10126 Torino, ITALY
www.cim.unito.it

Contact: silvio.aime@unito.it

Offered Technology (ies)
Magnetic Resonance Imaging (MRI) / Validation with: µCT / µPET / µPET/CT / µSPECT / Fluorescence-Mediated Tomography (FMT) / Bioluminescence Imaging (BLI) / Ultra Sound (µUS)

MRI assessment supported with: Responsive and targeting probes (Paramagnetic and CEST agents) / DCE for assessment of vascular permeability / Nanosized carriers (Liposomes, LDL, Ferritin) / Hyperpolarization (with para-H2)

Supports to Validation Studies : µCT: major X-ray Contrast Agents; µPET: FDG, Ga-68 probes; µSPECT: Tc-99m and In-111

Available resources
Animal facilities / Animal / Data processing and analysis / Housing facilities / Probe preparation

 

Facility Nr. 55
NMR and Molecular Imaging LaboratoryDepartment of General, Organic and Biomedical Chemistry,Universityof Mons,Avenue Maistriau, 19 7000 Mons, Belgium http://portail.umons.ac.be/ EN2/universite/facultes/fmp/services/service1/Page/default.aspx

Contact: robert.muller@umons.ac.be

Offered Technology (ies)
Magnetic Resonance Imaging (MRI) validated by Bioluminescence Imaging (BLI)
Synthesis and characterization of superparamagnetic particles for cellular labeling and molecular imaging  by MRI and validated by BLI

 

Available resources
Animal facilities
Animal preparation
Technical assistance to perform syntheses
Technical assistance to run instruments for characterizations

 

Facility Nr. 58
Uppsala University Faculty of Science and Technology, Dept of Biochemistry and Organic chemistry, Uppsala University; P.O.Box 576; 75122 Uppsala, Sweden

Contact: Prof Bengt Langstrom (Bengt.langstrom@biorg.uu.se); Prof Lars Baltzer (Lars.Baltzer@biorg.uu.se)

Offered Technology(ies)

Design, synthesis, method development, technology and characterization of molecular probes for biomedical research, especially radiotracers for PET and SPECT

Technical details: Novel technologies for radiolabelling using short-lived radionuclides based on microfluidics. Intrumentation for and consultation on in vitro evaluation of molecular structure, purification and affinity

Available Resources

Methodological setup (e.g. design of study protocol)
Training in infrastructure use
Masters and PhD programs
Probe preparation
Wet lab space
Material science and technology

Hub collaboration with the following sites

a) UU; The Tech. Science Faculty, The Angstrom Laboratory, Uppsala University, Dept Engineering Sciences, Box 534, 751 21 Uppsala;
http://www2.teknik.uu.se/mst

Contact:Prof. Klas Hjort (klas.hjort@angstrom.uu.se)

Offered Technology(ies)

Training in miniaturized systems for PET chemistry

Technical details: Microfluidics for micro reactors and radio organic chemistry, e.g., high pressures; gas and liquid flows; integrated fluidic actuators; heterogenous integration; filters; microwave devices

Available Resources
Clean room facility for microsystem technology
Backend line for miniaturized systems
Microactuator laboratory
Electron microscopy and materials analyses of miniaturized technologies
Thin film deposition systemas

b) Karolinska Institutet PET Centre; Karolinska Institutet, Department of Clinical Neuroscience, Psychiatry Section, 17176 Stockholm, Sweden

Contact: Prof Christer Halldin  (christer.halldin@ki.se)

Offered Technology(ies)
PET Radioligand development
SPECT
Preclinical PET CNS
Clinical PET CNS Research
Post mortem ARG human lab

Technical details: High Competence in PET Radioligand Development, GMP production of PET Radioligands for Clinical Use; Multimodality micro-PET/MR/CT small animal imaging facility, HRRT human PET camera with 1.5 mm resolution

Available Resources
Probe preparation
Methodological setup (e.g. design of study protocol)
Animal preparation
Animal facilities (rodents, monkeys)
Hot lab with 16 hot cells
ARG lab with phosphorimager
Wet lab space
Server Space
Data processing and analysis
Clinical studies

c) UU, Medical / Pharm faculty, Preclinical PET platform, Department of Medicinal Chemistry, Uppsala University
http://pet.medchem.uu.se

Contact: Prof Gunnar Antoni  (gunnar.antoni@pet.medchem.uu.se)

Offered Technology(ies)
µPET
µSPECT
µCT
Cyclotron and hotcells for production of radiotracers

Technical details: Competence in PET radiochemistry and tracer development, collaboration with Uppsala university hospital with access to GMP production laboratories for PET radiotracers and PET-CT

Available Resource
Technical assistance to run instrument
Methodological setup (e.g. design of study protocol)
Animal preparation
Animal facilities (rodents, monkeys)
Wet lab space
Data processing and analysis

d) Umeå University NUS PET/CT-Centrum, Radiation Scienes, Diagnostic Radiology, NUS, Umeå University, Sweden

Contact:Prof Katrine Riklund-Ahlstrom (Katrine.Riklund.ahlstrom@diagrad.umu.se)

Offered Technology(ies)
PET/CT

Technical details: Equipped for development and GMP production of 11C and 18F labelled tracers. radio-chemists with high competence in radiotracer development and production

Available Resources
Hot cells and dispensor. Chemistry systems for routine, kitbased chemistry and chemistry systems for in house developed chemistry. System for automatic injection of 15O-water available

 

PROBE DEVELOPMENT FOR ADVANCED LIGHT MICROSCOPY

 

Facility Nr. 47
Molecular Imaging Center, EMBL Heidelberg, Cell Biology & Biophysics Unit, Meyerhofstr. 1, 69117 Heidelberg, Germany
http://www.embl.de/research/units/cbb/schultz/

Contact: schultz@embl.de

Offered Technology (ies): Chemical Biology / Probe development

Technical Details: Synthetic organic chemistry, Fluorescent probes (FRET, translocation) for enzyme activities, metabolite and ion levels.

Available resources:
Chemistry lab
NMR
Purification equipment
High-end imaging
Biophysics core facility (ITC, fluorimeter, photometer, SPR, platereader)

 

Facility 15
Van Leeuwenhoek Centre for Advanced Microscopy (LCAM), PO-box 94215,1090 GE Amsterdam, The Netherlands
www.lcam.nl

Contact:Th.w.j.gadella@uva.nl or K. Jalink@nki.nl

Offered Technology(ies)
Genetic encoded probe development (1)
FRET-) biosensor  development (2)
Functional Imaging of Living Cells (e.g. FRAP, FRET) (3)
Fluorescence Lifetime Imaging Microscopy (FLIM) (4)
Fluorescence Correlation Spectroscopy (FCS) (5)

Technical details:
Ad 1. Saturated and targeted mutagenesis of  enhanced fluorescent proteins
Ad 2. Second messenger and translocation probes
Ad3. Specialized in high-content FRET
Ad 4. Frequency-domain wide-field and TCSPC-confocal
Ad5. Advanced GFP-based FCCS, PCH, FCLS options

Available Resources
Wetlab space (cloning, PCR, cell culturing, transfection, transformation; ML1, ML2, yeast, mice)
Screening (FACS, plate readers and lifetime/intensity screen)
Protein handling/purification (FPLC, affinity beads, gel & blotting)
Spectroscopy (fluorescence and absorbance spectrometers, single molecule spectroscopy (FCS and lifetime)).
Advanced microscopy lab, spectral confocals, FLIM, FCS