Advanced Light Microscopy-- General Access (WP6)

DENMARK

FINLAND

FRANCE

GERMANY

GREECE

HUNGARY

IRELAND

ITALY

NETHERLANDS

NORWAY

 PORTUGAL

POLAND

SPAIN

SWEDEN

 SWITZERLAND

UNITED KINGDOM

 

 

DENMARK

Facility Nr. 1
Danish Molecular Biomedical Imaging Center, University of Southern Denmark
Campusvej 55, DK-5230 Odense M, Denmark
www.dambic.dk

Contact:lagerholm@memphys.sdu.dk; bagatolli@bmb.sdu.dk

Offered Technology (ies)
Laser Scanning Confocal Systems
Spinning Disc Confocal Systems
Multiphoton Systems
Non-linear Microscopy Techniques (SHG)
Probe Microscopy (atomic force)

For technical details please see: www.dambic.dk

Available resources
Training in infrastructure use
Methodological setup (e.g. design of study protocol)
Wet lab space
Data processing and analysis


Facility Nr. 2
Center for Advanced Bioimaging (CAB) Denmark, University of Copenhagen, Thorvaldsensvej 40, DK-1871 Frederiksberg C, Denmark 
www.cab.ku.dk

Contact: als@life.ku.dk; cab@life.ku.dk

Offered Technology (ies)
Laser Scanning Confocal Systems
Spinning Disc Confocal Systems
Functional Imaging of Living Cells (e.g. FRAP, FRET)
High-throughput Microscopy
Multiphoton Systems

Upright confocals with dipping lenses
Inverted confocals with temp.- and CO2 controlled chamber
Automatic dispensing for High throughput Microscope

http://www.cab.ku.dk/Instruments.aspx

Available resources
Instruments
Technical assistance to run instrument
Methodological setup (e.g. design of study protocol)
Training in infrastructure use
Probe preparation

 

 

FINLAND

Facility Nr. 3
Turku BioImaging and Cell Imaging Core (CIC) at the Turku Centre for Biotechnology, Turku BioImaging, P.O.B. 123, FI-20521 Turku
http://www.bioimaging.fi

Contact: maritta.loytomaki@abo.fi; eeva.rainio@btk.fi;

Offered Technology (ies)
Laser Scanning Confocal Systems:
  a) standard confocal microscopy
  b) high-content live cell confocal imaging
  c) confocal imaging combined with atomic force microscopy
Functional live cell imaging (for example, FRAP, FRET, FLIM)
Multiphoton microscopy  for live cell and intravital imaging

For more technical details, please see here.

Available resources
Instruments are accessed through our own administration and resource management program “Asimov”
Technical assistance to run the instrument
Methodological setup (e.g. design of study protocol, help with probes etc.)
Basic cell culture facilities
Data processing and analysis including server space


Facility Nr. 4
HFIC HelsinkiFunctional Imaging Center with the sites:

Biomedicum Imaging  Unit (BIU); Instituteof Biomedicine, Universityof Helsinki; http://www.biu.helsinki.fi/
Contact: BIU Imaging Coordinator Anthony Squire (anthony.squire@helsinki.fi)

Light Microscopy Unit (LMU); Instituteof Biotechnology, Universityof Helsinki; http://www.biocenter.helsinki.fi/bi/lmu 
Contact: LMU Head of unit, Kimmo Tanhuanpää (kimmo.tanhuanpaa@helsinki.fi)

Electron Microscopy  Unit (EMU); Instituteof Biotechnology, University of Helsinki; http://www.biocenter.helsinki.fi/bi/EM
Contact: EMU Laboratory Director Eija Jokitalo (eija.jokitalo@helsinki.fi)

Offered Technology (ies)
Laser Scanning Confocal Systems
Correlated Light and Electron Microscopy
Functional Imaging of Living Cells (e.g. FRAP, FRET, TIRF, FLIM)
Stochastic Optical Reconstruction Microscopy (STORM)
Optical Projection Tomography (OPT)

Available resources
Instruments
Technical assistance to run instrument
Methodological setup (e.g. design of study protocol)
Training in infrastructure use
Wet lab space
Data processing and analysis

 


FRANCE

Facility Nr. 5
Paris Centre Imaging Center (PCIC)
(Paris XIII) Institut J. Monod www.ijm.fr/en/ijm/facilities/imagoseine
(Paris XV) Institut Pasteur www.imagopole.org
(Paris V) Ecole Normale supérieure www.biologie.ens.fr/dbmicpht et Institut Curie http://pict-ibisa.curie.fr

Contact: pcicheads.contact@gmail.com

Offered Technology(ies)
Fluorescence Correlation Spectroscopy (FCS & Co)
Total Internal Reflection Fluorescence Microscopy (azimuthal TIR, FRAP/TIRF/WF)
Photo-activated Localization Microscopy (PALM, SPT- PALM)
Correlated Light and Electron Microscopy
Spinning Disc Confocal Systems

Technical details: The PCIC gathers technologies available on three very close although distinct sites inside Paris, (south part); Development of new image processing and analysis software; Guidelines on common procedures of access and other technologies available are detailed on each websites of the facilities

Available resources
Technical assistance to run instrument
Data processing and analysis
Wet lab space
Server Space
Housing facilities

 

 

GERMANY

Facility Nr. 6
Life Imaging Center (LIC); Albert-Ludwigs-University Freiburg, Centre of Systems Biology Analysis (ZBSA)
http://www.imaging.uni-freiburg.de/

Contact:nitschro@zbsa.uni-freiburg.de

Offered Technology(ies)
Laser Scanning Confocal Systems
Spinning Disc Confocal Systems
Deconvolution widefield microscopy
Total Internal Reflection Fluorescence Microscopy (TIRF)
Multiphoton Systems
Functional Imaging of Living Cells (e.g. FRAP, FRET)

Technical details: See for instrumentation and infrastructure: http://www.imaging.uni-freiburg.de/facility.php

Specific technics for confocal and 2-photon microscopes see: www.focusonmicroscopy.org/2011/PDF/294_Nitschke.pdf:

Automated, multiposition Time-lapse of large areas by irregular tile acquisition and Time-lapse tracking of moving and growing samples
Automatic recognition of recording areas by user defined features
Long-time imaging of cultured cells in flow chambers

Available resources
Instruments
Technical assistance to run instrument
Training in infrastructure use
Probe preparation
Data processing and analysis
Training workstations
Housing facilities
Bio-safety L2


Facility Nr. 7
BioDIP – Biopolis Dresden Imaging Platform, Dresden, Germany
http://ifn.mpi-cbg.de/

Contact: lmf@mpi-cbg.de ; imaging@biotec.tu-dresden.de

Offered Technology(ies)
Laser Scanning Confocal Systems
Multiphoton Systems
Spinning Disc Confocal Systems
Deconvolution widefield microscopy
Total Internal Reflection Fluorescence Microscopy (TIRF)

Technical details: https://ifn.mpi-cbg.de/

Available resources
Instrumentation
Methodological setup
Instruments
Technical assistance to run instrument
Training in infrastructure use
Housing facilities

 

INTERNATIONAL ORGANIZATION

Facility Nr. 8
Advanced Light Microscopy Facility (ALMF), EMBL Heidelberg,
Meyerhofstr. 1, D – 69117 Heidelberg, Germany
http://www.embl.de/almf/almf_services/

Contact:almf@embl.de

Offered Technology(ies)
Total Internal Reflection Fluorescence Microscopy (TIRF)
Ground State Depletion Imaging
Multiphoton Microscopy
Fluorescence Correlation Spectroscopy (FCS)
Functional Imaging of Living Cells (e.g. FRAP, FRET)

Technical Details: Laser nanosurgery, Uncaging, DNA damaging/ repair

Available resources
Instrumentation
Methodological setup (e.g. design of study protocol)
Training in infrastructure use
Animal facilities (mouse, drosophila, zebrafish, c. elegans)
Wet lab space
Data processing and analysis
Housing facilities

 

 

GREECE

Facility Nr. 9
Greek BioImaging facility with 3 units:
(1)BioImaging Unit-(BRFAA), Biomedical Research Foundation Academyof Athens,
4 Soranou Efessiou Athens, 11527, Greece
www.bioacademy.gr/Faculty/core.php?cr=17

Contact: spagakis@bioacademy.gr

(2)Functional Imaging Unit (UP), Universityof Patras, School of Medicine, University Campus 26505 Rio, Patras, Greece
www.med.upatras.gr/en/Pages/department/facilities.aspx

Contact: lygerou@med.upatras.gr, taraviras@med.upatras.gr

(3)CIBIT Unit-(DUTH) , DemocritusUniversity of Thrace, Dep. Molecular Biology & Genetics, 68100 Alexandroupolis, Greece
www.biostrength.gr

Contact: mkoffa@mbg.duth.gr

Offered Technology (ies)
Laser Scanning Confocal Systems (1,2)
Spinning Disc Confocal system (3)
Deconvolution widefield microscopy  (1)
Total Internal Reflection Fluorescence Microscopy (TIRF) (3)
Functional Imaging of Living Cells (e.g. FRAP, FRET) (1,2,3)
Quantitative Calcium Imaging (1)

Consultation on sample prep; data acquisition, data processing and analysis.

Available resources
Instruments (1,2,3)
Technical assistance to run instrument (1,2,3)
Methodological setup (1,2,3)
Animal facilities (mice, rats, rabbits) (2)
Wet-lab space (2,3)

 

 

HUNGARY

Facility Nr. 10
Molecular Cell Analysis Core Facility; Department of Biophysics and Cell Biology, Medical and Health Science Center,University of Debrecen, 98 Nagyerdei krt., H-4032 Debrecen, Hungary
http://www.biophys.dote.hu/en/node/225

Contact:Director: Prof. János Szöllősi, szollo@dote.hu; Contact: Dr. György Vámosi, vamosig@dote.hu ; Electron microscopy: Prof. Miklós Antal, antal@chondron.anat.dote.hu

Offered Technology(ies)
Functional Imaging of Living Cells (e.g. FRAP, FRET)
Fluorescence Correlation Spectroscopy (FCS)
Laser Scanning Confocal Systems
Total Internal Reflection Fluorescence Microscopy (TIRF)
Correlated Light and Electron Microscopy

Technical details: Confocal laser scanning microscopes equipped with FCS/FCCS (Olympus FV1000, Zeiss LSM510 META); FRET techniques for confocal microscopy and flow cytometry (spectral, donor/acceptor photobleaching, two-sided); Laser scanning cytometry; Cell sorting; 4-laser Olympus TIRFM; Fast Ca2+ imaging (Zeiss LIVE), Spectrofluorimetry; AFM; Electron microscopy;  FLIM (from 2012)

Available resources
Technical assistance to run instrument
Methodological setup (e.g. design of study protocol)
Training in infrastructure use
Data processing and analysis
Housing facilities

 

 

IRELAND

Facility Nr. 11
National BioPhotonics & Imaging Platform Ireland :
(http://www.nbipireland.ie):
RoyalCollege of Surgeons in Ireland http://www.rcsi.ie
Focas Research Institute, Dublin Institute of Technology, http://www.focas.dit.ie
NBIPI@DCU, National Centre for Sensor Research, Dublin City University, Dublin 7, Ireland, http://www.nbip.dcu.ie/facilities_ncsr.html

Contact:lnbipadmin@rcsi.ie

Offered Technology (ies)
Laser Scanning Confocal Systems
Electron Microscopy
Functional Imaging of Living Cells using FRET
Raman and Infrared Spectroscopy
Fluorescence Lifetime Imaging Microscopy

For technical details see http://www.nbipireland.ie/equipment

Available resources
Instruments
Technical assistance to run instrument
Methodological setup (e.g. design of study protocol)
Data processing and analysis
Probe preparation (in Spectroscopic facilities)
Wet lab space
Server Space


Facility Nr. 12
National BioPhotonics & Imaging Platform Ireland :
(http://www.nbipireland.ie):
Centre for Microscopy and Imaging, National University of Ireland, Galway, Ireland.
http://imaging.nuigalway.ie

Contact: peter.owens@nuiglway.ie ; peter.dockery@nuigalway.ie

Offered Technology(ies)
Laser Scanning Confocal Systems
Spinning Disc Confocal Systems
Deconvolution widefield microscopy
Electron Microscopy

For technical details see http://www.nbipireland.ie/equipment/205-nuig

Available resources
Instruments
Technical assistance to run instrument
Training in infrastructure use
Methodological setup
Wet lab space
Server space
Data processing and analysis.

 

 

ITALY

Facility Nr. 13
Advanced Light and Electron Microscopy BioImaging Center (ALEMBIC); San Raffaele Scientific Institute & University DIBIT 1  ia Olgettina 58, 20132 - Milano – Italy
www.sanraffaele.org/research/alembic

Contact:grohovaz.fabio@hsr.it

Offered Technology(ies)
Laser Scanning Confocal Systems
Spinning Disc Confocal Systems
Deconvolution widefield microscopy
Functional Imaging of Living Cells (e.g. FRAP, FRET)
High-throughput Microscopy

Technical details: www.sanraffaele.org/research/alembic

Available resources
Instruments
Technical assistance to run instrument
Methodological setup
Training in infrastructure use
Animal facilities (rats, mouse)
Wet lab space
Server Space
Data processing and analysis
Training workstations

 

 

NETHERLANDS

Facility Nr. 14
Erasmus OIC (Optical Imaging Centre); Dept. of Pathology, Erasmus MC, PO-box 2040, 3000 CA Rotterdam
erasmusmc.nl/oic

Contact:g.kremers@erasmusmc.nl

Offered Technology(ies)
Functional Imaging of Living Cells (FRAP, FRET, time-lapse)
Laser Scanning Confocal Systems
4PI Microscopy
Fluorescence Correlation Spectroscopy (FCS)

Technical details: ad Functional Imaging of Living Cells:Quantitative FRAP analysis by computer modeling;Combined FRAP/FRET (donor-recovery-after-acceptor-bleaching); ad 4PI Microscopy: Sample preparation;

Available resources
Instruments
Technical assistance to run instrument
Methodological setup (design of study protocol)
Wet lab space
Data processing and analysis
Training in infrastructure use


Facility Nr. 15
Van Leeuwenhoek Centre for Advanced Microscopy (LCAM);
PO-box 94215, 1090 GE Amsterdam, The Netherlands
www.lcam.nl

Contact:Th.w.j.gadella@uva.nl

Offered Technology(ies)
Functional Imaging of Living Cells (e.g. FRAP, FRET) - Multimodal FRET and biosensor development
Fluorescence Lifetime Imaging Microscopy (FLIM) - Frequency-domain wide-field and TCSPC-confocal
Fluorescence Correlation Spectroscopy (FCS) - Advanced GFP-based FCCS, PCH, FCLS options
High-throughput Microscopy - Specialized in high-content FRET
Laser Scanning Confocal Systems - Several setups for multispectral, multiposition, prolonged low phototoxic imaging

Available resources
Instruments Technical assistance to run instrument
BioSafety L2
Wet lab & cloning, cell culturing and over-expression/ knockdown vector libraries
Support for cultured mammalian cells and plant work
Data processing and analysis

 

 

NORWAY

Facility Nr. 16
NorMIC-UiO; Univ Oslo. Dep Mol Biosciences and Radium hospital, Oslo University Hospital
http://www.mn.uio.no/imbv/english/research/about/infrastructure/imaging/imbv.html
Electron microscopy: http://www.ous-research.no/ucem/l

Contact:oddmund.bakke@imbv.uio.no

Offered Technology(ies)
Laser Scanning Confocal Systems
Spinning Disc Confocal Systems
Functional Imaging of Living Cells (e.g. FRAP, FRET)
Electron Microscopy

Technical details: Live cell imaging, Rapid FRAP/photoactivation on single organelles, Immuno Electron microscopy and standard

Available resources
Instruments
Technical assistance to run instrument
Methodological setup (e.g. design of study protocol)
Training in infrastructure use
Probe preparation

 

POLAND

Facility Nr. 17
Laboratory of Cell Biophysics, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University
ul. Gronostajowa 7, 30-387 Krakow, Poland
http://www.wbbib.uj.edu.pl/helios

Contact: Prof. Jerzy Dobrucki; jerzy.dobrucki@uj.edu.pl

Offered Technology (ies)
Laser Scanning Confocal Systems
Stimulated Emission Depletion Microscopy (STED)
Fluorescence Correlation Spectroscopy (FCS); Fluorescence Lifetime Imaging Microscopy (FLIM)
Functional Imaging of Living Cells (e.g. FRAP, FRET)

For technical details, info on sample preparation, appointments, microscopy, image analysis and cytometry literature, published work from this laboratory see: http://www.wbbib.uj.edu.pl/helios

Available resources
Instruments
Technical assistance to run instrument
Methodological setup (e.g. design of study protocol)
Training in infrastructure use
Housing facilities


Facility Nr. 18
Laboratory of confocal microscopy, Nencki Institute of Experimental Biology, Nencki Institute, Pasteura 3, 02-093 Warsaw, Poland
www.nencki.gov.pl/en

Contact: g.wilczynski@nencki.gov.pl

Offered Technology (ies)
1)  Laser Scanning Confocal Systems
2)  Stimulated Emission Depletion Microscopy (STED)
3)  Total Internal Reflection Fluorescence Microscopy (TIRF)
4)  Fluorescence Lifetime Imaging Microscopy (FLIM)
5)  Functional Imaging of Living Cells (e.g. FRAP, FRET)

Available resources:
Instruments, Technical, assistance to run instrument, Methodological setup (e.g. design of study protocol), Data processing and analysis

 

 PORTUGAL

Facility Nr. 60
IBMC, Rua do Campo Alegre 823, 4150-180 PORTO, Portugal 
www.imbc.up.pt

Contact: sampaio@ibmc.up.pt

Offered technology(ies)
Laser Scanning Confocal Systems
Spinning Disc Confocal Systems
Deconvolution widefield microscopy
Functional Imaging of Living Cells (e.g. FRAP, FRET)

For technical details see here.

Available resources
Instruments
Technical assistance to run instrument
Methodological setup
Training in infrastructure use
Wet lab space & Animal facilities (Drosophila, Mouse and Rat)
Data processing and analysis

 

SPAIN

Facility Nr. 19
Confocal Microscopy Unit, SpanishNational Cancer Research Center(CNIO), c/ Melchor Fernandez Almagro 28029 Madrid, Spain.
http://www.cnio.es

Contact: Diego Megias, dmegias@cnio.es

Offered Technology (ies)
Laser Scanning Confocal Systems
Multiphoton Systems
Functional Imaging of Living Cells (e.g. FRAP, FRET)
High-throughput Microscopy
White light laser

For technical details, see here.

Available resources
Instruments
Technical assistance to run instrument
Methodological setup, Data processing and analysis
Wet lab space
Data processing and analysis
Training workstations


Facility Nr. 20
Advanced Digital Microscopy, IRB Barcelona,
C. Baldiri Reixac, 10-12, 08028 Barcelona, Spain
http://adm.irbbarcelona.org/

Contact: microscopy@irbbarcelona.org

Offered Technology (ies)
High-throughput Microscopy 
Spinning Disk Confocal Systems with FRAP + Photoactivation
Laser Nanosurgery + FRAP
Single Plane Illumination Microscopy (SPIM) on a  Macroscope

For technical details, see here.

Available resources
Instruments
Technical assistance to run instrument
Methodological setup (e.g. design of study protocol)
Training in infrastructure use
Wet lab space
Server Space
Data processing and analysis
Training workstations

 

 

SWEDEN

Facility Nr. 21
Centre for Cellular Imaging, Sahlgrenska Academy, University of Gothenburg, Sweden
http://www.cf.gu.se/english/Centre_for_Cellular_Imaging/

Contact: cci@gu.se

Offered Technology (ies)
Laser Scanning Confocal Systems
Multiphoton System
Laser Microdissection & Pressure Catapulting
Correlated Light and Electron Microscopy
Fluorescence Correlation Spectroscopy (FCS)

Technical details: Consultation on sample prep; data acquisition, data processing and analysis.For detailed information on instrumentation, please go here.

Available resources
Instruments
Technical assistance to run instrument
Methodological setup
Wet lab space
Data processing and analysis
Animal facility (Mice)

 

 SWITZERLAND

Facility Nr. 61
EPFL-SV-PTECH-BIOP
Station 19
CH-1015 Lausanne
http://biop.epfl.ch/

Contact: Arne Seitz, arne.seitz@epfl.ch

Offered technologies:
Point scanning confocal micrscopy
Multiphotopn imaging
Live cell imaging (temp. and CO2 controlled chamber)
Functional imaging

For more technical details please see: http://biop.epfl.ch/INFO_facility_general.html

Available resources 
Methodological setup (e.g. design of study protocol, help with probes etc.)
Training to use the instrument
Technical assistance to run the experiment
Basic cell culture facilities
Data processing and analysis including server space

 

Facility Nr. 62
Center for Microscopy and Image Analysis, University of Zurich
http://www.zmb.uzh.ch

Contact: Urs Ziegler, ziegler@zmb.uzh.ch

Offered technologies
Deconvolution widefield microscopy
Laser Scanning Confocal Systems
Correlated Light and Electron Microscopy
Stimulated Emission Depletion Microscopy (STED)
Stochastic Optical Reconstruction Microscopy (STORM)

Available resources
Instruments
Housing facilities

Bio-safety L1 – L3

Wet lab space for specimen preparation including high pressure and plunge freezing, embedding, cryosectioning

 

UNITED KINGDOM

 

Facility Nr. 22
Beatson Advanced Imaging Resource (BAIR); Beatson Institute for Cancer Research, University of Glasgow
www.beatson.gla.ac.uk/BAIR

Contact: k.anderson@beatson.gla.ac.uk

Offered Technology (ies)
Mouse in vivo imaging using (1) multi-photon and (2) confocal microscopy to perform (3) photo-activation/bleaching, (4) collagen SHG, and (5) FLIM-FRET

Available resources
Instruments
Technical assistance to run instrumenT
Methodological setup
Training in infrastructure use
Probe preparation
Animal preparation
mouse facilities
Data processing and analysis


Facility Nr. 23
College of Life Sciences Light Microscopy Facility, University of Dundee http://microscopy.lifesci.dundee.ac.uk/omx

Contact: omx-admin@dundee.ac.uk

Offered Technology (ies)
3D Structured Illumination
Very fast 4 channel live imaging

Technical details: OMX microscope / 3 channel 3D Structured Illumination / 4 separate channels suitable for up to 90 fps / Consultation on sample prep; data acquisition, data processing and analysis /
See http://microscopy.lifesci.dundee.ac.uk/omx/links_resources.html

Available resources
Instruments
Technical assistance to run instrument
Methodological setup
Data processing and analysis