POLAND
Advanced Light Microscopy Node Poland
The Polish Advanced Light Microscopy (ALM) Node is a multi-sited, multimodal EuroBioimaging Node offering open access to instrumentation in biological imaging including multi-modal ALM, CLEM, EM, functional imaging, high-throughput microscopy and super-resolution microscopy. The ALM Polish Node provides full hands-on and theoretical training in all of the imaging techniques available at the Node facilities. We support in-house scientists and visitors in using microscopy methods, project planning, sample preparation, microscope selection and use, image processing and visualization. Our Node comprises two imaging facilities located in Warsaw at the Nencki Institute of Experimental Biology PAS and the Mossakowski Medical Research Centre PAS and one imaging facility in Cracow at the Faculty of Biochemistry, Biophysics and Biotechnology, Jegiellonian University.
Specialties and expertise of the Node
Expertise in the study of life at all major levels of organization, beginning at the molecular and elemental levels, through subcellular organelle dynamics, cellular and tissue-level processes, as well as the whole organism. The main fields of expertise: molecular interactions, protein dynamics, single molecule methods, intracellular processes in neuronal cells, energy metabolism (in cancer, cardiovascular, neurodegeneration), cell biology (nucleus, mitochondria, cytoskeleton, Golgi complex), physiology of the cell (intracellular membrane transport, membrane channels, apoptosis, regeneration stem cells, lymphoma cells, pharmacology).
Offered Technologies
| Technologies | Euro-BioImaging |
| Deconvolution widefield microscopy (DWM) | ✓ |
| Laser scanning confocal microscopy (LSCM/CLSM) | ✓ |
| Spinning disk confocal microscopy (SDCM) | ✓ |
| Structured illumination microscopy (SIM)* | ✓ |
| Total internal reflection fluorescence microscopy (TIRF) | ✓ |
| Two-photon microscopy (2P) | ✓ |
| Single Molecula localisation microscopy (SMLM) | ✓ |
| Stimulated emission depletion microscopy (STED) | ✓ |
| Quantitative Phase Imaging* (QPI) | ✓ |
| High throughput microscopy/high content screening (HTM/HCS) | ✓ |
| Fluorescence (cross)-correlation spectroscopy (FCS/FCCS) | ✓ |
| Fluorescence Resonance Energy Transfer (FRET) | ✓ |
| Fluorescence Recovery After Photobleaching (FRAP) | ✓ |
| Fluorescence Lifetime Imaging Microscopy (FLIM) | ✓ |
| Microdissection ** | ✓ |
| TEM of chemical fixed samples (TEM) | ✓ |
| EM tomography | ✓ |
| serial section TEM | ✓ |
| Immuno-gold EM on resin sections (resin-EM) | ✓ |
| Pre-embedding immunolabelling (pre-embed IL) | ✓ |
| Scanning Electron Microscopy (SEM) | ✓ |
| pre-embeded CLEM | ✓ |
Additional Services offered by the Node
- Instruments
- Methodological setup (e.g. design of study protocol and standard operation procedures)
- Technical assistance to run instrument
- Wet lab space and cell culture room
- Data processing and analysis
Instrument highlights
Special features of instruments at the Polish ALM Node:
- Availability of array of different imaging modalities, including: spectral, two-photon, time-resolved and super-resolution fluorescence microscopy
- The use of 3D high-resolution imaging with block-face Scanning electron microscopy (SEM)
Contact details
Olga Sumara, o.sumara@nencki.edu.pl
Office of International Relations and Project Management at the Nencki Institute of Experimental Biology PAS
Joanna Szczepanowska, j.szczepanowska@nencki.edu.pl
Professor at Nencki Institute of Experimental Biology Polish Academy of Sciences
Jędrzej Szymański, j.szymanski@nencki.edu.pl
Head of the Laboratory of Imaging Tissue Structure and Function at the Nencki Institute of Experimental Biology PAS
+4822 5892508