Erasmus MC OIC - Advanced Light Microscopy Rotterdam Node
The Erasmus Optical Imaging Centre (OIC) – Multimodal Imaging Node in Rotterdam is dedicated to the innovation, application and maintenance of a wide variety of optical imaging modalities (https://erasmusoic.nl/). An important focus of the OIC is to develop and implement innovative technology, mainly quantitative methods (largely, but not exclusively based on FRAP, FRET, FCS and time lapse imaging) to study living cells. This Node also has experience with molecular biology, tissue culture and cell biology, animal experimentation and drug delivery. The OIC provides high resolution (intracellular) imaging, stringent control over animal and tissue condition (for instance tight control of tissue temperature for hyperthermia studies), and expertise in intratumoral processes during drug delivery and tumor manipulation.
- Laser scanning confocal microscopy (LSCM/CLSM)
- Multiphoton systems
- Spinning disc confocal microscopy (SDCM)
- Total internal reflection fluorescence microscopy (TIRF)
- Fluorescence correlation spectroscopy (FCS)
- Fluorescence resonance energy transfer (FRET)
- Fluorescence recovery after photobleaching (FRAP)
- 4Pi Microscopy (4PI)
- Photo activated localization microscopy (PALM)
- Stochastic optical reconstruction microscopy (STORM)
The OIC offers the capability to do combined STORM/PALM super-resolution imaging. This can also be combined with laser scanning confocal microscopy. This system is also equipped with an incubator for live cell super-resolution imaging. Two confocals are equipped with a 355nm and/or 266nm deep-UV laser for localized subcellular manipulation for studying DNA repair and organelle healing. Spinning disc confocal system and TIRF microscope are equipped with ultra-fast FRAP unit for localized subcellular manipulation.
Prof. Dr. A. B. Houtsmuller
Scientific director of Erasmus Optical Imaging Centre / Professor of Functional Cell Anatomy