Facility of Multimodal Imaging - AMMI Maastricht

The Advanced Microscopy and Molecular Imaging (AMMI) Nodes connect state-of-the-art (light and electron) microscopy with high-end, innovative molecular imaging technologies. It aims to assist academic and industrial users performing fundamental and applied studies in biomedical and molecular imaging. As such, AMMI offers access to a translational, interdisciplinary research program in leading international expertise centers, combining research and education. The AMMI infrastructure contains both basic and high-end/one-of-a-kind imaging techniques on microscopic and macroscopic level combined with an extensive palette of non-invasive imaging techniques.

Specialties and expertise of the Maastricht Node

Our facility connects the newest imaging instrumentation within socially relevant clinical and biomedical research areas. A broad palette of research topics is covered within and resulting from collaborations with the world-leading research schools CARIM (Cardiovascular Disease), NUTRIM (Nutrition and Translational Research in Metabolism), GROW(Oncology and Developmental Biology) and MHeNS (Mental Health and Neuroscience).
Keywords are cardiovascular research, atherosclerosis, oncology, development, metabolism, and brain.
Due to extensive joined projects between these research schools and the world leading Institutes MERLN (Technology-Inspired Regenerative Medicine), M4I (Maastricht for Imaging), and the biochemical company DSM, we have broad experience regarding imaging in the areas of biomaterials and biomass as well. For an overview of Schools and Institutes and their specific research topics, we refer you to the webpage. Selecting the specific school or Institute brings them to their homepage. Finally, through a strong collaboration with Uniklinikum in Aachen, we also offer the possibility, after discussions, to use various microscopes available there (animal whole body fluorescence imaging, Lightsheet, STORM, Airy confocal).

Offered Technologies:

  • Laser scanning confocal microscopy (LSCM/CLSM)
  • Various systems including white light or Ca2+-imaging
  • Spinning disc confocal microscopy (SDCM)
  • Stimulated emission depletion microscopy (3D-STED)
  • Multi-photon (MP) systems
  • Correlative light electron microscopy (CLEM)
  • Fluorescence-lifetime imaging microscopy (FLIM on MP)
  • Fluorescence resonance energy transfer (FRET on MP)
  • Fluorescence recovery after photobleaching (FRAP)
  • High Throughput Microscopy
  • Electron microscopy (EM)
  • 3T MRI (clinical)
  • 7T MRI (both animal and clinical)
  • 9.4T MRI (clinical)
  • CT-PET
  • microCT
  • micro ConeBeam CT
  • microPET
  • microMRI/MRS
  • microSPECT
  • microUS
  • Optical Imaging (fluorescence and bioluminescence)
  • Image-guided small animal irradiator

Additional services offered by the Node

  • Instruments
  • Technical assistance to run instruments
  • Methodological setup (e.g. design of study protocol and standard operation procedures)
  • Training in infrastructure use
  • Probe preparation
  • Animal breeding, Animal ethical licenses, Animal experiment protocols, and animal handling
  • Wet lab space
  • Data storage and analysis
  • Artificial Intelligence, Deep learning and Radiomics for data analysis
  • Training workstations
  • Training seminar room

Instrument highlights

With the broad range of techniques, we offer, we have created an imaging platform, ranging from EM, via super-resolution STED, confocal, and multi-photon microscopy, towards whole body imaging. This platform connects a broad range of microscopic and macroscopic imaging techniques. Some of the EM and LM techniques are located in the Microscopy Core Lab (MCL) run by Dr. C. Lopez-Iglesias.

3D rendered image of an ex vivo carotid artery, mounted on perfusion chamber (see reference) under pressure. The carotid artery underwent wire-injury, was mounted, followed by ex vivo injection of ultrasound microbubbles (Mbs) targeted to VCAM-1 (3 days after wire-injury). The MBs are rendered fluorescent by labeling them with rhodamine. Additional co-staining using antibody against PECAM-1 (green, endothelial cells) VCAM-1 (grey) and Syto41 (blue, cell nuclei) were performed. For more experimental details and the biological context see Curaj, A., et al., Noninvasive molecular ultrasound monitoring of vessel healing after intravascular surgical procedures in a preclinical setup. Arteriosclerosis, thrombosis, and vascular biology, 2015. 35(6): p. 1366.

Contact details

Marc A.M.J. van Zandvoort
Professor in Advanced Microscopy
+31 (0)43 3881361

Ludwig Dubois
Associate Professor Precision Medicine
+31 (0)43 388 2909

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