French BioImaging Node

The France BioImaging Node is at the crossroads between molecular and cell biology, integrated physiology, biophysics and engineering, mathematics and informatics. This unique coordinated infrastructure in the European landscape gathers several large facilities and R&D laboratories specializing in imaging in 5 National Geographic locations and one transversal Node in bioimage informatics. FBI aims at creating the most efficient adoption of the latest advances in technologies and methods related to microscopy, by the users of the imaging facilities. R&D labs agree to open their technologies and expertise to the European community either by hosting users on their own site or after technological transfer to the FBI core facilities. These technologies and methods, reinforced by a strong support in computational analysis, provide quantitative measures and integrative understanding of a wide range of cell and tissue activities in biological models, from the simplest organism, to small animals in normal and pathological situations.

Offered Technologies:

ISIDORe is a Horizon Europe funded project that brings together 154 partners from 32 countries around the world, and is designed to effectively support research on infectious diseases and increase preparedness for pandemic.

Deconvolution widefield microscopy
Laser scanning confocal microscopy (LSCM)
Multiphoton systems
Spinning disc confocal systems
Total internal reflection fluorescence microscopy (TIRF)
Fluorescence correlation spectroscopy (FCS)
Fluorescence cross-correlation spectroscopy (FCCS)
Fluorescence-lifetime imaging microscopy (FLIM)
Fluorescence resonance energy transfer (FRET)
Fluorescence recovery after photobleaching (FRAP)
Raman Spectroscopy
High Throughput Microscopy
Electron microscopy
Correlative light electron microscopy (CLEM)
Digital scanned laser light-sheet fluorescence microscopy (DSLM)
Objective-coupled planar illumination (OCPI)
Optical projection tomography (OPT)
Selective plane illumination microscopy (SPIM)
Ground state depletion microscopy (GSD)
Photo activated localization microscopy (PALM)
Reversible saturable optical fluorescence transitions (RESOLFT)
Stimulated emission depletion microscopy (STED)
Stochastic optical reconstruction microscopy (STORM)

Instrument highlights

Among a number of special features offered by the France-BioImaging Node let us quote:

  • Specialization in super resolution in the Bordeaux site with more than ten systems covering STED, GSD, PALM/STORM and combination of methodologies dedicated to Neurosciences and plant imaging projects
  • Imaging equipment in P3 labs for host-pathogens studies in Paris sites
  • Preclinical-biomedical imaging by innovative approaches in Marseille and Paris sites

Additional services offered by the Node

  • Instruments
  • Methodological setup (e.g. design of study protocol and standard operation procedures)
  • Technical assistance to run instrument
  • Probe preparation
  • Animal preparation
  • Animal facilities
  • Wet lab space
  • Data processing and analysis
  • Training seminar rooms
  • Housing facilities
  • Regulatory affairs management service
  • Biobanking, biological material storage and processing

Contact details

Jean Salamero (DR1 CNRS, PhD)
Head of the National Infrastructure in Biomedical Science( INBS) France BioImaging

33 (0) 1 56 24 64 49

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