Fluorescence Lifetime Imaging (FLIM)

Fluorescence-lifetime imaging microscopy (FLIM) is an imaging technique for producing an image based on differences in the fluorescence-lifetime rather than its intensity. By quantifying variations in the exponential decay rate of the fluorescence from a fluorescent sample (fluorescence-lifetime) it is possible to report on molecule proximity, pH changes and even polarity. It can be used as an imaging technique in confocal microscopy, two-photon excitation microscopy and multiphoton tomography. Since the fluorescence-lifetime is insensitive to changes in fluorophore intensity or concentration, it is the most quantitatively precise technique to report on fluoresce resonance energy transfer (FRET).

We use cookies to manage and improve the services of the Euro-BioImaging Web Portal. To find out more, read our Privacy policy.
Please note: For best experience we do not recommend using Internet Explorer.