ITALY

Advanced Light Microscopy Italian Node


The Italian ALM Node is a large multi-modal and multi-sited Node that comprises five imaging facilities located in Naples, Genoa, Padua, Florence and Milan. These facilities have a long experience of mutual interaction and collaboration. The Node is coordinated by the Institute of Biochemistry and Cell Biology, Naples, an institute belonging to the National Research Council of Italy. The Node provides open-access imaging services to both academia and industry and serves about 1000 users annually. While each location provides access to a wide range of technologies, they also specialize in one or two specific technologies as listed below. The Node offers a complete service package starting from sample preparation and imaging to final image analysis, quantitation and visualization.

Specialties and expertise of the Node

The facilities of this Node offer a broad range of services but they specialize in particular in the following technologies and research areas:

Technologies

  • Naples: Correlative light electron microscopy with 3D imaging, correlative microscopy with FRET imaging
  • Genoa: Super-resolution imaging
  • Padua: Functional imaging
  • Florence: Non-linear microscopy, functional imaging
  • Milan: Correlative microscopy with 3D tomography, elemental imaging, high-throughput microscopy

Research applications

  • Naples: Cell biology, membrane trafficking, signaling, cancer biology
  • Padua: Signaling, neurobiology
  • Milano: Neurobiology, cell biology, signaling, pharmacology
The member groups also have experience with a wide spectrum of model systems including mammalian cell culture systems, insect cell culture systems, and model organisms like Drosophila and mouse

Offered Technologies:

  • Deconvolution widefield microscopy
  • Laser scanning confocal microscopy (LSCM)
  • Multiphoton microscopy systems
  • Spinning disc confocal microscopy (SDCM)
  • Total internal reflection fluorescence microscopy (TIRF)
  • Fluorescence correlation spectroscopy (FCS)
  • Fluorescence-lifetime imaging microscopy (FLIM)
  • Fluorescence resonance energy transfer (FRET)
  • Fluorescence recovery after photobleaching (FRAP)
  • Raman Spectroscopy
  • High Throughput Microscopy
  • Electron microscopy
  • Selective plane illumination microscopy (SPIM)
  • 4Pi Microscopy
  • Ground state depletion microscopy (GSD)
  • Photo activated localization microscopy (PALM)
  • Stimulated emission depletion microscopy (STED)
  • Stochastic optical reconstruction microscopy (STORM)

Additional services offered by the Node

  • Support in experimental design
  • Technical assistance to use the microscopes
  • Practical training courses
  • Cell culture facilities
  • Wet lab space
  • Animal facilities
  • Data processing and analysis

Instrument highlights

The members of the Italian ALM Node are also involved in the development of novel imaging methods, instrumentation and reagents. These include:

  • Naples: FRET-based methods to detect large multi-molecular complexes, Raman microscopy, image analysis methods and microfluidics
  • Genoa: various aspects of super-resolution microscopy
  • Padua: probes for functional imaging of intracellular ions and metabolites
  • Florence: functional imaging and Raman imaging
  • Milano: High-throughput microscopy

Contact details

Alberto Luini
Coordinator of the Italian ALM Node
alberto.luini@ibbc.cnr.it
+39 081 6132 535
Seetharaman Parashuraman
Head, Bioimaging facility, IBBC-CNR
raman@ibbc.cnr.it
+39 081 6132 283

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