Wageningen Imaging and Spectroscopy Hub (WISH) - ALM and Molecular Imaging Node Wageningen

The WISH – ALM and Molecular Imaging Node in Wageningen joins the outstanding scientific and technical excellence of four participating expertise groups specialized in light microscopy, microspectroscopy, magnetic resonance and electron microscopy. This Node applies advanced imaging technologies to plant research within a multidisciplinary research environment of cell & developmental biologists, (bio-) physicists and biochemists. Advanced instrumentation has been developed for the particular purpose of studying photosynthesis in vivo with the use of ultrafast fluorescence at the level of individual cells and chloroplasts.

Specialties and expertise of the Node

Spectroscopic investigations of energy transfer within photosynthetic complexes; functional imaging of membrane associated protein complexes involved in plant signaling and photosynthesis; and the visualization of plant developmental processes like cell division.

Offered Technologies:

  • Laser scanning confocal microscopy (LSCM)
  • Multiphoton systems
  • Spinning disc confocal systems (SDCM)
  • Total internal reflection fluorescence microscopy (TIRF)
  • Fluorescence correlation spectroscopy (FCS)
  • Fluorescence-lifetime imaging microscopy (FLIM)
  • Fluorescence resonance energy transfer (FRET)
  • Fluorescence recovery after photobleaching (FRAP)
  • Fluorescence cross-correlation spectroscopy (FCCS)
  • Photo activated localization microscopy (PALM)
  • Stochastic optical reconstruction microscopy (STORM)
  • Electron microscopy

Contact details

Johannes Hohlbein
Associate Professor, Laboratory of Biophysics
Wageningen University & Reserach


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