Cryo Scanning Electron Microscopy (Cryo-SEM)*

Cryo-SEM is an excellent technique for imaging of liquid and semi-liquid materials surfaces, hydrated biological specimens (e.g. biofilms, extracellular matrix, hyphae, etc.) in close-to native state. In this method, a beam of focussed ions is scanned across the sample surface (SEM) under cryogenic conditions. In combination with the high-pressure freezing and freeze fracturing/etching techniques, inner structures of samples can also be observed

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