Light sheet microscopy is a rapidly emerging technology that combines optical sectioning with multiple-view imaging to observe tissues and living organisms with impressive resolution. Three-dimensional imaging in light-sheet-based microscopy is performed by moving the specimen through the light sheet in small steps and recording a two-dimensional image at each step. In DSLM, 3D imaging can alternatively be performed by moving the light sheet through the specimen and by displacing the detection lens accordingly. In multiple-view (or multi view) imaging, the same volume inside the specimen or even the entire specimen is recorded along several angles. The resulting multiple-view information can be combined into a single image stack by data post-processing using a fusion algorithm.

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