i3S Course: Optical Microscopy Imaging for Biosciences



March 20–24, 2023


Porto, Portugal

14th edition 20-24 March 2023 | 9:30h to 18:30h | i3S, Porto 

i3S, part of our Portuguese Platform of BioImaging Node is organising the 14th edition of the Course on Optical Microscopy Imaging for Biosciences. This course offers the participants a comprehensive light microscopy course that explores the fundamentals and introduces the state-of-art of advanced fluorescence microscopy techniques. The course will flow from sample preparation to quantitative image analysis. It will include theoretical lectures taught by specialists in the field and hands-on practical modules supported by major microscopy companies where the participants will have the opportunity to work with high-end microscopy technology. 

Target audience: The course is designed for Ph.D. students, early career researchers plus all researchers interested in acquiring skills in optical microscopy. 

Course Contents:

  • Specimen preparation; 
  • Light properties and image formation; 
  • Optical contrast techniques in transmission light microscopy; 
  • Widefield Fluorescence microscopy
  • Optical sectioning methods: Deconvolution, Laser scanning confocal, two-photon, TIRF and Lightsheet microscopy; 
  • Live cell imaging; 
  • Analyzing biomolecular interactions and dynamics: FRET, FLIM, FRAP, etc…; 
  • Super-resolution microscopy; 
  • Artificial Intelligence in microscopy;
  • Quantitative image analysis. 

For more information and registration: https://www.i3s.up.pt/training-detail.php?v=253

Early Bird Registration | Until 6th February 2023

Program Flyer: