Bridging Scales: Unveiling the Mechanical Properties of Biomaterials and Organoids Using AFM and Bulk Rheology

Understanding the mechanical characteristics of biological samples, engineered tissues, and organoids is essential for developing advanced three-dimensional models that closely replicate native tissue and organ functions. Such characterisation enhances the physiological relevance of in vitro systems and supports efforts to reduce dependence on animal models in biomedical research. This course, organised by i3s, University of Porto, part of Euro-BioImaging's PPBI Node, will help you to do so via imaging.

This course provides comprehensive training in the use of Atomic Force Microscopy (AFM) and bulk rheology to investigate the viscoelastic properties of biomaterials across nano- and macroscales. Participants will learn to measure parameters such as stiffness, adhesion, Young’s modulus, viscous modulus, and viscosity, without the need for prior sample preparation. Additionally, the course introduces high-throughput nanoindentation techniques integrated with imaging tools for screening complex 3D tissue models.

By the end of the course, participants will be able to:

• Apply AFM for nanoindentation and dynamic mechanical analysis
• Learning how to properly calibrate the AFM to ensure accurate and reliable force measurements
• Understand core mechanical parameters (e.g., stiffness, Young’s modulus)
• Select the appropriate models and software for data processing and analysis
• Use rheological methods (oscillatory shear, viscometry)
• Be acquainted with high-throughput nanoindentation integrated with imaging and with its potential for mechanical properties mapping.

Target audience: Postdoctoral researchers, PhD, MSc and BSc students; life sciences researchers, technical staff, members of technology centers and industry professionals.

Course duration: 21 hours (over 3 days)

Course Format: In person | Includes both theoretical and practical components

Course requirements: Laptop

Language: English

Course organizers:
Manuela Brás (i3S), Ricardo Vidal (i3S), and Isabel Amaral (i3S)