Live Cell Imaging Microscopy Course

Date: 2023-01-30 to 2023-02-17

From January 30-February 17, the Live Cell Imaging core facility at KI will run its yearly microscopy course: Improve your imaging skills – from sample preparation to image analysis.

All the course lectures will be broadcasted live on Zoom, free of charge and there is no need to register. On this page, you can find the course schedule (public activities are in blue) and the Zoom link to join.

The aim for this course is to improve the microscopy skills of students and researchers who have already used a microscope to acquire digital images of fluorescent samples but feel that more knowledge could help them.

The course covers the following topics:

  • •Optics, image formation
  • •Fluorescence, fluorophores
  • •Bleedthrough
  • •Anatomy of a microscope
  • •Objectives and refraction index
  • •Cameras and detectors
  • •Noise and background, Bit depth and saturation
  • •Multichannel imaging and spectral unmixing
  • •Resolution and contrast
  • •Sample preparation, Immunostaining
  • •Nyquist sampling
  • •Confocal and wide field settings
  • •Speed, High throughput/content
  • •Volume imaging, deconvolution
  • •Clearing and expansion
  • •Live cell imaging
  • •Fourier
  • •AI, Super Resolution microscopy
  • •Colocalization
  • •Data handling, OMERO.figure

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