Tech Exchange with Proteintech

When

October 24, 2025 14:00–14:30 CEST

Presented by

Philipp Becker

Get to know our latest Industry Board member Proteintech and learn all about antibodies and nanobodies for microscopy.

Philipp Becker will present on "Seeing More: Nanobodies and FlexAble Antibody Labeling Kits in Fluorescence Imaging".

Figure 1: Immunofluorescence analysis of HeLa cells co-stained with mouse IgG1 anti-HSP60 antibody (66041-1-Ig) and mouse IgG2b anti-Lamin A/C antibody followed by Nano-Secondary® alpaca anti-mouse IgG1, recombinant VHH, CoraLite® Plus 555 (smsG1CL555-1, yellow) and Nano-Secondary® alpaca anti-mouse IgG2b, recombinant VHH, CoraLite® Plus 647 (smsG1CL647-1, magenta). Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

Figure 2: Immunofluorescence analysis of HeLa cells co-stained 5 different rabbit IgGs. Ki-67 (MA5-14520) FlexAble 2.0 CoraLite Plus 405 (Cyan); CD147 (11989-1-AP) FlexAble 2.0 CoraLite Plus 488 (Green); Lamin B1 (12987-1-AP) FlexAble 2.0 CoraLite Plus 555 (Yellow); Coilin (10967-1-AP) FlexAble 2.0 CoaraLite Plus 647 (Red); Tubulin (80713-1-RR) FlexAble 2.0 CoraLite Plus 800 (Magenta); Images were taken on a Olympus confocal microscope.

Abstract
The more we want to know, the more we need to see. Every imaging technique has its limitations, but every limit can be pushed, with the right tools. VHHs (Nanobodies) are uniquely suited to achieve the highest possible resolution. Their small size minimizes epitope-label displacement, significantly improving localization precision in fluorescence microscopy. Beyond resolution, nanobodies provide excellent specificity, improved tissue penetration, and compatibility with live-cell imaging, making them powerful alternatives to conventional antibodies. In this talk, I will discuss how nanobodies expand the imaging toolbox, from multiplexing to super-resolution, and how their unique properties help us push the boundaries of resolution and image quality. Further, I will explore Proteintechs unique FlexAble Antibody Labeling technology with you.