Correlative Light and Electron Microscopy of Plant tissues


September 7, 2023
AgroServ France-BioImaging Plant Biology User Forum

Imaging technologies support research into the structure and function of plants, shed light on plant health, resilience and adaptability, and help answer agroecology-related research questions. The Euro-BioImaging User Forum “Understanding Plant Biology” will showcase how imaging supports cutting edge research in this domain and provide information on funding opportunities for agroecology-related research projects via the AgroServ project. At this event, Clement Chambaud, CNRS, will explain how correlative light and electron microscopy in collaboration with the Bordeaux Imaging Center, part of France BioImaging, supports his research on plant tissues.  Full abstract below.

What: Euro-BioImaging User Forum “Understanding Plant Biology”

When: October 12, 2023, from 14:00-17:00 CEST

Where: Online

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ABSTRACT

Correlative Light and Electron Microscopy of Plant tissues

Clement Chambaud, CNRS

Bordeaux Imaging Center, France BioImaging

Correlative Light and Electron Microscopy (CLEM) is a powerful imaging method that consists in combining for the very same cell or tissue area observations of light and electron microscopy. Lately, there is a growing interest in the application of CLEM in biology, especially for yeast and mammal samples, but plant applications remain scarce. Various workflows of CLEM exist and depend on the type of sample and biological questions. In this talk, Clement Chambaud will focus an approach called In Resin Fluorescence (IRF) that was adapted for plant tissues. It allows preserving the fluorescent properties of conventional fluorescent proteins during the sample preparation steps for transmission electron microscopy that, in order to have the best ultrastructural preservation possible, are usually incompatible with fluorescence preservation. CLEM protocols are always a matter of compromise between fluorescence preservation and ultrastructure. IRF for plant was adapted for roots and hypocotyl of Arabidopsis thaliana and combined with high-resolution imaging in 3D by electron tomography. It has enable us to gain insight into understanding plasmodesmata biogenesis during plant grafting, autophagy or phase separation. Here, we will develop the bottlenecks of this approach, from plant sample preparation, handling and mounting for fluorescence to transmission electron microscopy and electron tomography observations.


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