Soft X-ray tomography showcasing


Published April 3, 2023
Technology
Category

Analysis of three-dimensional biological cell samples is critical for understanding the mechanisms of viral disease and for developing novel therapeutics. Cryo soft X-ray tomography (cryo-SXT) is the unique technology that can image whole intact cells in 3D under normal and pathological conditions without labelling, at high throughput and high spatial resolution.  It is a promising approach with many possible applications from cell and developmental biology to virology. This new technology successfully completed the Euro-BioImaging showcasing process for new technologies, highlighting the open access readiness of the technology and broad user interest.

Soft X-ray tomography requires strong photonic illumination which is traditionally only available at synchrotron labs. However, the system showcased here is a laboratory-scale Soft X-ray microscope, developed by SiriusXT. Soft X-ray tomography is performed on cryo-fixed samples without the need for additional labelling and the SXT-100 can accept samples either on TEM grids or in capillaries, smoothing the way for application of both SXT and EM on the same sample and allowing the correlation of both techniques.

Do you think cryo Soft X-ray Imaging could be a good approach for your research or your facilities' user base? Learn more about this technology and discover some specific case studies for virus and infection biology, in the full showcasing report by Sirius XT.

Bringing new technologies to the imaging community

To support the imaging community and its associated high-quality research, Euro-BioImaging must remain at the technological forefront. Showcasing is the first step in this workflow to ensure that new technologies are continuously integrated by the community into the Euro-BioImaging portfolio. For more information please visit this page.

It is aimed at demonstrating:

a) User need and relevance for the scientific community (through e.g. documentation by technology hosts on successful external user access to this technology; submitted letters of interest from users; relevant research publications associated with this technology in general, etc.)

b) An operational access model for external users to this technology (through documentation of access model by technology hosts).


More news from Euro-BioImaging

Muriel Mari Masterclass EVOLVE Mentoring

July 10, 2025

Women in Science: Redefining the Room - EVOLVE Masterclass with Dr. Muriel Mari 

As part of the EVOLVE Mentoring Masterclass series, Euro-BioImaging hosted Dr. Muriel Mari (Aarhus University) on May 27, 2025 for an honest and powerful…

Photo by McCoy Wynne and the Centre for Cellular Imaging at the University of Liverpool.

July 10, 2025

Congratulations to the Euro-BioImaging UK Node! 

The UK Euro-BioImaging Node has received a £1.8 million strategic award from UKRI-BBSRC and UKRI-MRC to expand access to cutting-edge imaging technologies for UK…

July 7, 2025

Fond memories of ELMI2025

The annual meeting of the European Light Microscopy Initiative (ELMI) took place at EMBL Heidelberg from June 3rd to 6th. As a conference representing…