PPBI in Focus: Behind the Scenes at the GIMM Bioimaging Platform

During a recent visit to one of the Portuguese Platform of Bioimaging (PPBI) Node facilities, Daniela Aviles, Scientific Project Manager at Euro-BioImaging, had the opportunity to explore the GIMM Bioimaging Platform, located at the Gulbenkian Institute of Molecular Medicine (GIMM) in Lisbon. She was warmly welcomed by José Rino, Head of the Facility, Gaby Martins, Advanced Training and Scientific Officer, Patricia Rodrigues, Imaging Specialist, and the rest of the dedicated team.

The visit provided a unique example into how a Euro-BioImaging Node transforms cutting-edge microscopy infrastructure into meaningful research support for life science investigators at all levels. 

From the outset, it was clear that the GIMM Bioimaging Platform is much more than a collection of high-end instruments. It functions as a comprehensive support ecosystem, guiding researchers from the initial stages of experimental design through to publication. The team of imaging specialists delivers tailored training, hands-on assistance, and expert advice in image acquisition, processing, and analysis—making the platform a true partner in discovery.

Advanced Technology and Resources

The platform boasts over 30 advanced imaging systems, supporting a wide range of research applications, including:

• Point-scanning and spinning disk confocal microscopy
• Multiphoton,light sheet microscopy and OPT
• Widefield and live-cell imaging, including fully automated high-throughput microscopy
• Slide scanning
• Advanced image analysis and processing workstations

This diverse instrumentation enables researchers to explore biological systems from subcellular resolution to whole organoid or tissue-level imaging—whether capturing static snapshots or dynamic, real-time processes.

The platform’s official website (GIMM Bioimaging Wiki) provides transparent, detailed information about system capabilities, operational protocols, and user training resources. Users can find documentation on key systems, such as:

• 3x ZEISS LSM 980 with Airyscan 2 – ideal for spectral unmixing and 3D imaging
• ZEISS Celldiscoverer 7 with LSM 900 – fully automated, equipped with a robot arm, incubator and liquid handler, perfect for live-cell high-throughput time-lapse confocal or widefield imaging
• 3x 3i Marianas SDC – spinning disk confocals designed for high-speed live-cell imaging

Training and Collaboration at Its Core

One of the most impressive features of the GIMM Bioimaging Platform is its emphasis on user empowerment. Beyond daily hands-on training, the platform regularly organises courses and workshops that cover everything from fundamental microscopy techniques to cutting-edge image analysis strategies.

What truly sets the platform apart is its collaborative spirit. Imaging experts actively engage with users throughout experimental design, protocol optimization, and data interpretation, making the platform an integrated part of the research process. This mentorship not only enhances reproducibility but also helps researchers maximize the potential of advanced imaging technologies.

A Key Player in National and European Imaging Infrastructures

The GIMM Bioimaging Platform plays a pivotal role within both the national and European imaging landscapes:

• As part of Euro-BioImaging ERIC, it supports transnational access to cutting-edge technologies and expertise.
• As a Node of the Portuguese Platform of Bioimaging (PPBI), it contributes to a coordinated national network of imaging excellence.
• As a ZEISS labs@location Partner, it is connected to a global network of microscopy centers, facilitating specialised services and direct industry collaboration.
• As part of the QUAREP-LiMi, GloBIAS and SPAOM, it actively participates in these communities’ working groups and activities.

These partnerships reinforce the platform’s position as a hub for technical innovation, knowledge sharing, and scientific collaboration across institutional and national boundaries.

The GIMM Bioimaging Platform showcases what it means to be a truly integrated imaging facility within Euro-BioImaging. With its powerful combination of advanced technology, expert support, and a deep commitment to user training and collaboration, the platform stands as a shining example of imaging excellence in Europe.

For scientists across disciplines seeking to elevate their research with state-of-the-art microscopy, the GIMM Bioimaging Platform offers not only exceptional tools but also the expert guidance and partnership needed to make every image count.