Synchrotron radiation phase contrast imaging for the 3D study of organoids: preliminary experience at Elettra

We will host the Special Edition Virtual Pub "Imaging Organoids" on Friday, January 30, from 1-3 pm CET. It will focus on methods used to image organoid systems. As part of this event, Elena Longo (Elettra) and Beatrice Anfuso (International Centre for Genetic Engineering and Biotechnology (ICGEB)), will give a talk entitled "Synchrotron radiation phase contrast imaging for the 3D study of organoids: preliminary experience at Elettra" (description below). Join us to hear this and other exciting presentations introducing a range of methods used to image organoid systems - from lung buds to mini-brains.

Description of the talk

Title: Synchrotron radiation phase contrast imaging for the 3D study of organoids: preliminary experience at Elettra

Authors: Elena Longo (Elettra) and Beatrice Anfuso (International Centre for Genetic Engineering and Biotechnology (ICGEB))

Synchrotron radiation phase contrast imaging is a powerful option to morphologically characterize complex biological systems. Specifically, X-ray phase-contrast tomography (XPCT) offers the advantageous 3D perspective to virtually evaluate specimens’ inner structures without any extensive sample manipulation. Here, we present a preliminary organoids imaging experience carried out at the SYRMEP imaging beamline of Elettra synchrotron, known as the Phase Contrast Imaging Node of Euro-Bioimaging. In this framework, we will illustrate the experimental pipeline adopted for the 3D study of different liver model organoids with sub-micrometer voxel size.